ABSTRACT
Drug-induced liver injury (DILI) is a major cause of acute liver failure in clinic.Numerous emerging cell-based models and high-throughput screening technologies are being applied to hepato?toxicity evaluation in vitro.Before applying these emerging technologies and approaches,it is important to determine which endpoints should be included in the testing system to improve the predictive power of DILI. Based on the important mechanisms of DILI, this review introduces the endpoints that can be used for in vitro evaluation of DILI,such as hepatotoxicity,mitochondrial toxicity,cholestatic,liver injury caused by active metabolites,and immune-based liver injury.The application of these endpoints in DILI detection systems and the challenges in the application process are summarized so as to provide refer?ence for in vitro evaluation of hepatotoxicity in the early stage of new drug development.